Cell bio lab report Essay

PurposeDuring this experiment we compared the hem agglutination answer of control find out A solution at 2 mg/ml in teach A buffer with the hemagglutination answer of your own purified study A sample that you diluted previously at 2 mg/ml in Con A buffer. The purpose of this lab was to fix the strength of the reaction by performing serial dilutions on both(prenominal) the Con A sample and the control Con A sample, and tick off through and through observations whether or non addition of brain sugar or mannose leave behind inhibit this reaction. I hypothesize that the Con A + galactose solutions leave alone have partial tone agglutination and partial no agglutination, and the Con A + mannose solutions will have every(prenominal) no agglutination.ResultsRows A and B had half(prenominal) agglutination and half no agglutination, while row C had all no agglutination. Row D had half agglutination and half partial agglutination, while row E had 4 columns with agglutination and 8 columns with no agglutination. Row F had complete agglutination throughout. Con A reaction plate (Row/Column)ReactionA1-A6 (Control) agglutination/ holdA7-A12 (Control)No agglutination/not inhibitB1-B6 (Con A + galactose)Agglutination/inhibitedB7-B12 (Con A + galactose)No Agglutination/not inhibitedC1-C12 (Con A + mannose)No Agglutination/not inhibitedD1-D5 (Sample)Agglutination/inhibitedD6-D12 (Sample)Partial agglutination/inhibitedE1-E4 (Con A + galactose)Agglutination/inhibitedE5-E12 (Con A + galactose)No Agglutination/not inhibitedF1-F12 (Con A + mannose)No agglutination/not inhibitedG1-G12 ((-)Control)Partial agglutination/inhibitedH1-H12 (RBCs)Partial agglutination/inhibitedDiscussionMy hypothesis was proven correct, the Con A + galactose solutions did have partial agglutination and partial no agglutination, and the Con A + mannose solutions did have all no agglutination. This means that in the Con A + galactose solutions the ones that had agglutination the red cells aggr egated with the lectin and depositary in the form of a uniform layer that covered the full bottom of the well including the slopes, and in the Con A + mannose solutions the ones that failed to have agglutination take place covered only part of the bottom. We found through this experiment that the control Con A sample and the Con A sample had very similar strengths of reaction.ConclusionIn this experiment through serial dilutions on a 96 well plate we immovable the strength of the each reaction on both the Con A sample and the control sample. We found through our experiment that the addition of galactose or mannose will partially inhibit or inhibit the reaction between our Con A sample and control Con A sample.