Alcohol Consumption Drosophila Melanogaster

Alcohol expending in sexually prevent output go drosophila melanogaster Ankit Sharma April 8, 2013 BIO 534 Introduction The growth wing drosophila melanogaster show addiction-like behavior towards inebriant where the move seem to be physiologically dependent on (Atkinson et. al. , 2012). The flies can thus exhibit a cognitive dependence where such behavior is stored in memory and resembling behavior is also shown in the future. This addiction is link to NPY neuropeptide levels which can be found in Drosophila where these neuropeptides serve as feeding stimulants (Shen et. al. , 2005).Alcohol consumption rate is higher when these NPY neuropeptide levels are suppressed (Wilcox, 2012). Thus, such factors are linked to the idea that fruit flies consume food that contains alcohol more than regular food, and such behavior increases over time (Devineni and Heberlein, 2009). Sexual frustration is also linked to increase in consumption of alcohol. Fruit flies consumed less alcohol when they were sexually satisfied because sex raised NPF levels and the flies consumed more alcohol when they were sexually frustrated because their NPF levels were suppressed (Azanchi et. l. , 2012). The purpose of the test is to determine if sexually frustrated antheral fruit flies Drosophila melanogaster resort to alcohol after being rejected by females. Thus, the sample will determine if the number of sexually frustrated fruit flies will be higher where there is alcohol in the surround than non-sexually frustrated fruit flies. The hypothesis to be tested is useless hypothesis. The null hypothesis states that sexual frustration has no effect on alcohol consumption by male fruit flies.Thus, there will not be a significantly higher number of rejected male fruit flies in an alcohol environment than non-sexually frustrated flies. Materials and Methods twain plastic cylindrical ampules were used to place wingless wild emblem fruit flies. The vials contained same amount of medi um for food. Both vials contained regular medium for food. The fruit flies were anesthesized and 40 males and 40 females were separated. 20 males were placed in a vial with 20 females that were decapitated by a razor blade. 20 separate males were placed in a different vial with 20 females.The experiment was conducted over a period of 4 days. dickens more vials were made, both containing 21% alcohol on one fractional side of the medium which was the closest percentage available compared to 15% fermentation alcohol used in other experiments. The males with decapitated females were transferred to one of the vials with alcohol. The males with regular females were transferred to the other vial with alcohol. The number of flies present on alcohol and public side of the medium was recorded in both vials for 20 legal proceeding in 1 minute increments. Three trials were conducted.The mean numbers of fruit flies present on the cardinal sides of both vials were measured. The mean was c alculated by the summation of the number of fruit flies in distributively sides of the vials and divided by 120. The null hypothesis was tested by conducting a two-tailed t-test. Results For all the number of fruit flies observed in the two vials (n=120) there was a significant difference in the number of fruit flies in the two vials in the (t=4. 87, df=118, p